In order to examine the initial appearance and development of the steroid producing cells (SPCs) during the process of ovarian differentiation, histology and ultrastructure of tilapia ( Sarotherodon niloticus ) ovaries were investigated from 10 to 50 days after hatching. In gonads of fry at 23–26 days after hatching, initial ovarian differentiation was confirmed by the differentiation of stromal aggregations in the proximal and distal region of the gonad on the side facing the lateral wall. This represents the initial formation of the ovarian cavity. At the same time as ovarian differentiation, a few large cells appeared initially in the vicinity of blood vessels. They have some of the ultrastructural features characteristic of SPCs such as a moderate number of mitochondria with tubular cristae, a large amount of smooth endoplasmic reticulum and many free ribosomes. Based on these ultrastructural criteria, together with the present finding that these cells further differentiated into the typical SPCs at older stages, these cells were identified as SPCs. Thereafter, by 30–50 days, SPCs increased gradually in number in the area enclosing the blood vessels of ovaries. The increase in SPCs coincided with the development of germ cells, including the multiplication of oogonia and the transformation from oogonia to oocytes.
A: This nm thick section through the tomogram of mito 1 is near the edge (20 nm distance) of the reconstructed 3D model shown in Figure 2 , top left. The cristae labeled A, B, and C correspond to the lamellar regions of the green cristae of Figure 2 , top left. A crista junction is seen in one crista (arrowhead). The red crista of Figure 2 is labeled (R). The open arrows indicate areas where the cristae membranes are in close proximity to the IBM. B: This deeper section into the tomogram is from the level where the crista junction of the red crista (R) of Figure 2 opens to the IBM via a crista junction (arrowhead). C: An enlargement of the previous figure that demonstrates a contact site between the outer mitochondrial membrane and the IBM (between arrowheads). D and E: These two sections ( nm thick) are from a tomogram of an example of the lamellar association form of the cristae. The close apposition of the cristae membranes that form the lamellar association is apparent. The rather consistent spacing between the lamellae is also found between the IBM and the first crista of the lamellar association (between arrows). D is close to the edge of the tomogram and thus near the edge of the 3D reconstruction of this mitochondrion in Figure 4 , top left. The first and second lamellae, starting at the IBM, are separate here. Also, the beginning of the crista junction from crista 3 (dark blue crista of the 3D model in Figure 4 ) is seen opening to the IBM (arrowhead). E: Deeper into the tomogram (10 nm distance), there is the beginning of a connection between cristae 1 and 2 (between arrows) and crista junctions are seen from cristae 3 and 4 (dark blue and yellow cristae of Fig. 4 ; arrowheads).
Dr Mercola has articles stressing the damage soy can do unless it is fermented which is only a few soy products. Soy milk and edamame are not on the list of safe soy as they are not fermented. Both are damaging to gut lining and, according to his research articles, hormone disruptive. Since he is careful to have extensive research and studies supporting his study, this is very conflicting. I often work with women that have major hormone issues. It’s troublesome to see two highly respected doctors -Dr Mercola and Dr Greger at opposite ends of the spectrum on soy -unless I am missing a component in this info vs Dr Mercolas?